Fish, who won his previous match on Louis Armstrong Stadium in five sets against Arnaud Clement, fell in the fourth round to fourth seed Novak Djokovic, who had played repeatedly on Ashe during the tournament.
"I haven't played on that court in two years. Man, is it different from playing on Louis Armstrong and Grandstand," said Fish. "There's not hardly any wind outside, and it's windy in there, really windy. For him to play every match in there and sort of get used to that, I think certainly helped him.
"Doesn't mean that if I play Clement out there that I win today by any means," added Fish, who lost in straight sets. "But it took me a while to get used to it."
After losing in the third round to Mikhail Youzhny a day earlier, Isner said, "I didn't hit a ball on that court, no practice or anything prior to this match. Same with my opponent, Mikhail," said Isner. "But without a doubt, had I been a little bit more comfortable on that court it probably would have helped, but it was the same for both of us. He handled it better."
Andy Roddick, a former champion and No. 9 seed, played both of his matches on Ashe stadium during the tournament, but the lower-seeded Americans had been fixtures on Armstrong and the Grandstand until the weekend. Querrey will play on Ashe for the first time in his fourth round match against Stanislas Wawrinka on Tuesday.
"Not a huge fan of the scheduling this week," Querrey said after his previous win. "We have a lot of Americans here. None of us play on center court. If you go to the French Open, they have [Richard] Gasquet, [Julien] Benneteau, [Gael] Monfils, they're on center court every day."