Rosol, who came in ranked 35th, was beaten 6-3, 4-6, 7-6 (5), 6-7 (4), 6-4 Monday by Germany's Julian Reister.
Their match was on tiny Court 19, a short walk away from Centre Court, where the then 100th-ranked Rosol pulled off a surprising five-set victory over two-time Wimbledon champion Nadal in 2012's second round. Nadal then took a seven-month break from the tour because of a left knee injury.
Against Reister, Rosol played the same sort of high-risk game he used so successfully against Nadal. This time, Rosol was not nearly as accurate, accumulating 74 unforced errors, 30 more than Reister.